Please follow the instructions listed in this fact sheet when handling etbr. There is an existing crystal structure in which ethidium bromide is complexed with a ribo cpg dinucleotide. View the stained gel using a standard transilluminator 302 or 312 nm and image the gel using an ethidium bromide filter. Ethidium bromide solution sc286960 hazard alert code key. Ethidium bromide is not regulated as hazardous waste at low concentrations, but is treated as hazardous waste by many organizations. Safety data sheet northwest missouri state university. Ethidium bromide, 1% solutionmolecular biology revision date 18jan2018 sensitivity to mechanical impactno information available sensitivity to static dischargeno information available specific hazards arising from the chemical keep product and empty container away from heat and sources of ignition.
Liquid ethidium bromide waste can be collected in the same container as the solid waste. Ethidium bromide etbr, a dark red, crystalline, nonvolatile powder that is moderately soluble in water, is widely used in research laboratories as a visualizing agent for nucleic acids. The fluorescence of ethidium bromide in aqueous solution is significantly lower than that of the interchelated dye. The toxic effects of etbr may be experienced if swallowed, inhaled, or absorbed through the skin. It fluorescents a redorange color under ultraviolet uv light and with increased fluorescence when bound to doublestranded dna. Pubmed comprises more than 26 million citations for biomedical literature from medline, life science journals, and online books. Your new online msds binder is a place for you to store the material safety data sheets. Ethidium reemits this energy as yelloworange light centered at 590 nm. Ethidium bromide is used to visualize dna in agarose gels and in many cesium chloride gradient protocols. Chemical product and company identification section 1.
That said, when mum brought back some veinasbestos samples from. Ethidium bromide is a toxic chemical and a mutagen. Contains tracking dyes and ethidium bromide, so gel loading buffer and ethidium bromide staining are not required. Ethidium bromide is not a regulated hazardous material, but due to its potential health hazards. Ethidium bromide is dnas and rnas intercalators, which makes it get into the empty area of the dual. Ive read somewhere about adding ethidium directly to the samples to be run on gels prior to running. Due to its unique structure, it can easily intercalate into dna strand. Exposure information commercial availabili production and producers. Material safety data sheet ethidium bromide solution sdsmsds. Therefore, it is commonly used as nucleic acid fluorescent tag in various techniques of the life science field. Adding ethidium directly to samples prior to loading apr052008 hi everyone, ive read somewhere about adding ethidium directly to the samples to be run on gels prior to running. The user must determine suitability of this information for his application. Ethidium bromide etbr is a wellknown fluorescent tag usually applied in molecular biological techniques like agarose gel electrophoresis.
Ethidium bromide is not a regulated hazardous material, but due to its potential health hazards, precautions should be taken during handling and disposal. Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. Ethidium is the fluorescent compound widely used in experimental cell biology and biochemistry to reveal doublestranded dna and rna. Do not dispose of ethidium bromide as biohazardous waste. Environmental precautions prevent further leakage or spillage if safe to do so. Ethidium bromide safe handling and disposal procedures. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy. Ethidium bromide is a potent mutagen used as a nucleic acid stain. Standard operating procedure template for ethidium bromide.
Ethidium bromide etbr is a mutagen, a suspected carcinogen and teratogen, and at high concentrations is irritating to the eyes. Ethidium bromide ethidium bromide is an intercalating agent which resembles a dna base pair. Are other fluorescent tags used instead of ethidium. Ask most biologists about handling it, and youre get a fearful expression and advice to use gloves, etc. Extreme high moderate low section 1 chemical product and company identification product name ethidium bromide solution. The mechanism of action for such compounds is known, in which these compounds are able to bind to the kinetoplastid. I am trying to minimize background while having nice sharp bands for microarray rtpcr validations. Wipe the area with a damp cloth after the work with ethidium bromide powder is complete.
But a msds for sybr safe reports a ld50 for rats of 5gkg, which is higher. Ethidium bromide intercalates inserts itself between nucleic acid base pairs and exhibits fluorescence under ultraviolet. Safety data sheet creation date 08jun2010 revision date 18jan2018 revision number 4 1. The detection limit of dna bound to ethidium bromide is 0. It has a role as an intercalator and a fluorochrome. Ethidium bromide safe handling, disposal, and emergency procedures. Citations may include links to fulltext content from pubmed central and. Ethidium bromide intercalates inserts itself between nucleic acid base pairs and exhibits fluorescence under ultraviolet light, providing molecular biologists with an excellent stain for visualizing and imaging nucleic acids. No warranty, express or implied, is made and labchem inc. The disposal of laboratory ethidium bromide remains a controversial subject. As ethidium bromide is carcinogenic, there are several biological and environmental problems are associated with it. Glow dyes have been specifically formulated for nucleic acid. What is the alternative for ethidium bromide in gel. Ethidium bromide laboratory spill and waste handling procedures.
Sodium bromide 6 information in this msds is from available published sources and is believed to be accurate. Load samples and run the gels using your standard protocol. The solution can be used to detect both doublestranded and singlestranded dna. The 10 mgml ethidium bromide stock solution can be diluted for gel staining or used as provided for csc1 gradient protocols. Dec 19, 20 ethidium bromide etbr is a wellknown fluorescent tag usually applied in molecular biological techniques like agarose gel electrophoresis. A safer alternative is to purchase ethidium bromide in solution. While preparing agarose gels why is ethidium bromide added. Bring information safety data sheet about ethidium bromide to medical center with you. The powder form is considered an irritant to the upper respiratory track, eyes and skin. Feb 27, 2020 ethidium bromide is a potent mutagen which has been commonly used as a nucleic acid stain for many years. Ethidium bromide etbr wastes are not regulated by the state of massachusetts or the epa. The powder is used to make a stock solution of 10 mgml, and the working concentration for gelstaining is 1 gml. The flat structure of ethidium bromide allows it to intercalate, or insert, between nitrogenous bases of a dna molecule. These filters use ionexchange resins and activated charcoal to remove the ethidium bromide from.
Product name ethidium bromide, 1% solutionmolecular biology cat no. Ethidium bromide is found in pretty much every molecular biology lab around. Material should be handled according to the material safety data sheet msds. A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium bromide is a dna intercalator, inserting itself between the base pairs in the double helix. The more common use of the ethidium bromide stain is the detection of dna or rna gel samples. Ethidium bromide etbr is the most commonly used nucleic acid stain for page or agarose gel electrophoresis. The crystal or powder form is odorless and appears dark red in color. It fluoresces a redorange colour under ultraviolet light and with increased fluorescence when bound to doublestranded dna. Ethidium bromide is a potent mutagen, often used in laboratories to stain nucleic acids in electrophoresis gels. Ethidium bromide is typically in powder or solution form and is soluble in water. Ethidium bromide has uv absorbance maxima at 300 and 360 nm, and an emission maximum at 590 nm. Ethidium monoazide is a fluorescent photoafinity label that, after photolysis, binds covalently to nucleic acids both in solution and in cells that have compromised membranes.
Ethidium bromide has uv absorbance maxima at 300 and 360 nm, and an emission maximum at 590. Ethidium bromide solution 10 mgml catalog or product number. Wear gloves and a face mask when working with ethidium bromide in powdered form. Apr 18, 2016 ethidium bromide is found in pretty much every molecular biology lab around. Ethidium has several experimentally useful properties including binding to nucleic acids. For staining a gel after electrophoresis, dilute a sample of the stock solution to 0. Accidental release measures personal precautions wear respiratory protection. Ethidium bromide etbr is commonly used as a nonradioactive dna stain to iden. Identification product name ethidium bromide, 1% solutionmolecular biology cat no. Wear appropriate protective eyeglasses or chemical safety goggles as described by oshas eye and face protection regulations in 29 cfr 1910. Rna sample loading buffer, with etbr scbt santa cruz. Ethidium bromide fact sheet environmental health and safety. A uv light can be used to determine complete inactivation. Citations may include links to fulltext content from pubmed central and publisher web sites.
Otherwise, aqueous solutions must be deactivated beforedisposal. Ethidium bromide is known as an intercalating agent. Ethidium bromide is an intercalating or stacking dye that enters the purine and pyrimidine base pairs. An acute exposure to ethidium bromide causes irritation of the mouth, upper respiratory tract, skin, and eyes. The 10 mgml ethidium bromide stock solution can be diluted for gel staining or used as. Ethidium bromide is typically purchased in powder or solution form and is soluble in water.
Ethidium bromide is strongly mutagenic, causing living cell mutations. Ethidium bromide laboratory spill and waste handling. Eb was reported by watkins 1952 to be prepared from 3. Ethidium bromide solutions and buffer solutions containing ethidium bromide should be filtered, decontaminated, or destroyed using one of the methods below or collected as chemical waste. Handling when pure ethidium bromide is used, handling should be performed in a fume hood wearing full protection clothing including a lab coat, closedtoe shoes, chemical resistant gloves and chemical safety goggles. Uses advised against not for food, drug, pesticide or biocidal product use details of the supplier of the safety data sheet emergency telephone number chemtreco, inside the usa. It fluorescents a redorange color under ultraviolet uv light and with increased. The fluorescence of etbr increases 21fold upon binding to doublestranded rna and 25fold on binding doublestranded dna so that destaining the background is not necessary with a low stain concentration 10. Ethidium bromide solution, molecular grade 10mgml, is a fluorescent dye suitable for staining nucleic acids after electrophoresis or in cesium chloride gradients. The fluorescence of ethidium monoazide is weak, but the intensity increases 15fold on binding to dna with excitationemiss.
This eliminates the hazards of working with the powdered form. Ethidium bromide waste disposal fact sheet environmental. Adding ethidium directly to samples prior to loading. Extreme high moderate low section 1 chemical product and company identification product name ethidium bromide solution statement of hazardous nature considered a hazardous substance according to osha 29 cfr 1910. Material safety data sheet section 1 ethidium bromide 1 chemical product and company identification msds name. Product ethidium bromide solution, 10ml product code h5041 substance number description amount symbols h504 ethidium bromide solution 1 t. Rna sample loading buffer, with etbr ethidium bromide, 50gml is formulated for use in rna sample preparation for denaturing formaldehydeagarose gel electrophoresis. Thats because the compound is used to make dna fluoresce when running gels, and it does that by slipping neatly betw. It is preferable to use ecofriendly flourescent dyes such as.
Are other fluorescent tags used instead of ethidium bromide. The fluorescence of etbr increases 21fold upon binding to doublestranded rna and. Typically eb is purchased in powder or solution form and is soluble in water. Ethidium bromide is dnas and rnas intercalators, which makes it get into the empty area of the dual helix. The glow dyes are designed to minimize the use of ethidium bromide and the risk associated with the regular use of ethidium bromide. What is the alternative for ethidium bromide in gel electrophoresis. After inactivation, the solution, devoid of ethidium bromide,can be poured down the drain with copious amounts of water.
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